mouse anti ena (Developmental Studies Hybridoma Bank)

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Developmental Studies Hybridoma Bank mouse anti ena
Mouse Anti Ena, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+ena/anti-Enabled/bio_rxiv__2025__09__12__675872-224-82-85
Average 93 stars, based on 5 article reviews

mouse anti ena - by Bioz Stars, 2026-09

93/100 stars

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Western Blot:

Article Title: Drosophila Atg9 regulates the actin cytoskeleton via interactions with profilin and Ena
Article Snippet: Transgenic lines were established by Bestgene. .. Antibodies Primary antibodies used in this study were rat anti-Atg9 (WB 1:300) [ 20 ], rat anti-HA (IF 1:150, WB 1:1500, Roche, 11867431001), mouse anti-tubulin (1:2000, DSHB, AA12.1), mouse anti-Ena (IF/WB 1:40, DSHB, 5G2), mouse anti-Profilin (IF 1:10, DSHB, chi1J), rat anti-DE-cadherin (IF 1:10, DSHB, DCAD2) rat anti-actin (WB 1:5000, Babraham Technix, BT-GB-237P), mouse anti-Flag M5 (WB 1:1000, Sigma, F4042), mouse anti-Fasciclin II antibody (1:500, DSHB, 1D4). .. The following secondary antibodies were used: goat anti-mouse Alexa Fluor 488 (A-11001), goat anti-mouse Alexa Fluor 546 (A-11003), goat anti-mouse Alexa Fluor 660 (A-21055), goat anti-rabbit Alexa Fluor 488 (A-11008), goat anti-rabbit Alexa Fluor 546 (A-11010), goat anti-rabbit Alexa Fluor 660 (A-21074), goat anti-rat Alexa Fluor 488 (A-11006), goat anti-rat Alexa Fluor 546 (A-11081)—all purchased from Invitrogen and biotinylated goat anti-mouse antibody (1:500, Jackson, 115-065-003).

Article Title: Drosophila Atg9 regulates the actin cytoskeleton via interactions with profilin and Ena
Article Snippet: Transgenic lines were established by Bestgene. .. Primary antibodies used in this study were rat anti-Atg9 (WB 1:300) [ 20 ], rat anti-HA (IF 1:150, WB 1:1500, Roche, 11867431001), mouse anti-tubulin (1:2000, DSHB, AA12.1), mouse anti-Ena (IF/WB 1:40, DSHB, 5G2), mouse anti-Profilin (IF 1:10, DSHB, chi1J), rat anti-DE-cadherin (IF 1:10, DSHB, DCAD2) rat anti-actin (WB 1:5000, Babraham Technix, BT-GB-237P), mouse anti-Flag M5 (WB 1:1000, Sigma, F4042), mouse anti-Fasciclin II antibody (1:500, DSHB, 1D4). .. The following secondary antibodies were used: goat anti-mouse Alexa Fluor 488 (A-11001), goat anti-mouse Alexa Fluor 546 (A-11003), goat anti-mouse Alexa Fluor 660 (A-21055), goat anti-rabbit Alexa Fluor 488 (A-11008), goat anti-rabbit Alexa Fluor 546 (A-11010), goat anti-rabbit Alexa Fluor 660 (A-21074), goat anti-rat Alexa Fluor 488 (A-11006), goat anti-rat Alexa Fluor 546 (A-11081)—all purchased from Invitrogen and biotinylated goat anti-mouse antibody (1:500, Jackson, 115-065-003).

Article Title: Drosophila Atg9 regulates the actin cytoskeleton via interactions with profilin and Ena.
Article Snippet: Transgenic lines were established by Bestgene. .. Primary antibodies used in this study were rat anti-Atg9 (WB 1:300) [20], rat anti-HA (IF 1:150, WB 1:1500, Roche, 11867431001), mouse anti-tubulin (1:2000, DSHB, AA12.1), mouse anti-Ena (IF/WB 1:40, DSHB, 5G2), mouse anti-Profilin (IF 1:10, DSHB, chi1J), rat anti-DEcadherin (IF 1:10, DSHB, DCAD2) rat anti-actin (WB 1:5000, Babraham Technix, BT-GB-237P), mouse anti-Flag M5 (WB 1:1000, Sigma, F4042), mouse anti-Fasciclin II antibody (1:500, DSHB, 1D4). .. The following secondary antibodies were used: goat anti-mouse Alexa Fluor 488 (A11001), goat anti-mouse Alexa Fluor 546 (A-11003), goat anti-mouse Alexa Fluor 660 (A-21055), goat anti-rabbit Alexa Fluor 488 (A-11008), goat anti-rabbit Alexa Fluor 546 (A-11010), goat anti-rabbit Alexa Fluor 660 (A-21074), goat anti-rat Alexa Fluor 488 (A-11006), goat anti-rat Alexa Fluor 546 (A-11081)—all purchased from Invitrogen and biotinylated goat anti-mouse antibody (1:500, Jackson, 115- 065-003).

Next-Generation Sequencing:

Article Title: A Diaphanous and Enabled dependent asymmetric actin cable array repositions nuclei during Drosophila oogenesis
Article Snippet: .. Antibody/label incubations were in PBS plus 0.1% Triton X-100 and 1% NGS using 1:500 Alexa488-Phalloidin (ThermoFisher, A12379), 1:1000 Hoechst (Invitrogen, 33342), and the following antibodies: 1:1000 mouse anti-Ena (DSHB, 5G2) and 1:1000 rabbit anti-Dia (gift from Wasserman lab, UCSD). ..

Generated:

Article Title: Rapid apical actin filament turnover is required for non-centrosomal microtubule organization in epithelium
Article Snippet: .. Primary antibodies used were rabbit anti-Capt (1:400, generated in the lab), mouse anti-acetylated αtubulin (1:100, Santa Cruz biotechnology 6-11B-1), rabbit anti-αTubulin (1:50, Abcam Ab18251), mouse anti-βTubulin (1:50, DSHB E7), mouse anti-Shot (1:25, DSHB mABRod1), mouse anti-Cnx99A (1:25, DSHB Cnx99A 6-2-1), mouse anti-Golgin84 (1:25, DSHB Golgin84 12-1), mouse anti-DyneinHC (1:25, DSHB 2C11-2), rabbit anti-Cad99C (1:2000, gift from Christian Dahmann lab; Schlichting et al ., 2005 ), mouse anti-profilin (1:25, DSHB chi 1J), Twinfilin (gift from Tapio Heino; Wahlström et al ., 2001 ), mouse anti-Ena (1:25, DSHB 5G2), rat anti-Tropomyosin (1:25, DSHB BB5/37.1) and rabbit anti-Phospho-Myosin II (1:300, Cell Signalling Technologies). .. Secondary antibodies, donkey anti-mouse Alexa 647 and goat anti-rabbit Alexa 647 (from Thermofisher Scientific), were used in a 1:300 dilution.

Staining:

Article Title: The Drosophila Actin Regulator ENABLED Regulates Cell Shape and Orientation during Gonad Morphogenesis
Article Snippet: .. Antibody staining was conducted as described previously , using the following primary antibodies: mouse anti-Ena (5G2, 1∶200; C. Goodman, DSHB), rabbit anti-Vas (1∶20,000; Lehmann, R.), chick anti-Vas (1∶300; Lehmann, R.), rabbit anti-Zfh-1 (1∶4,000; Lehmann, R.), rabbit anti-Serpent (1∶500; D. Hoshizaki), rabbit anti-GFP (1∶1,000; Clontech), rat anti-DE-cad (DCAD2, 1∶50; T. Uemura, DSHB). ..

Incubation:

Article Title: Toll receptors mediate tissue intrinsic surveillance against aberrant cells by detecting cell fate aberrations
Article Snippet: .. Primary antibody incubation was performed in 400 μl PBT overnight at 4 °C, using the following antibodies (see also ): mouse anti-dorsal (1:10, DSHB-7A4), mouse anti-Ena (1:100, DSHB-5G2), mouse anti-Ptc (1:20, DSHB-Apa1), mouse anti-Wg (1:100, DSHB-4D4-s), rabbit anti-Dcp-1 (1:400, Cell Signalling-9578S), rabbit anti-GFP (1:400, ThermoFisher-G10362), rabbit anti-mNeonGreen (1:2000, Abcam-ab321887), rat anti-E-cadherin (1:100, DSHB-DCAD2-s), rat anti-RFP (1:2000, Chromotek-5F8), mouse anti-β-gal (1:2000, Promega-Z3783), rabbit anti-HA (1:200, Invitrogen-715500), rat anti-HA (1:250, Roche-3F10). .. Secondary antibody and dye incubation was performed in 400 μl PBT for 2 h at 22°C with the following antibodies and dyes: goat anti-rabbit Alexa Fluor 488 (1:500, Invitrogen-A11008), goat anti-rabbit Alexa Fluor 555 (1:500, Invitrogen-A21428), goat anti-rat Alexa Fluor 555 (1:500, Invitrogen-A21434), goat anti-mouse Alexa Fluor 555 (1:500, Invitrogen-A21422), goat anti-rabbit Alexa Fluor 647 (1:500, Invitrogen-A21244), goat anti-mouse Alexa Fluor 647 (1:500, Invitrogen-A32728), donkey anti-rat Alexa Fluor 555 pre-adsorbed (1:500, Abcam-AB150154), donkey anti-mouse Alexa Fluor 647 pre-adsorbed (1:500, Abcam-AB150111), DAPI (0.25ng/μl, Sigma), Phalloidin-Alexa Fluor 405 (1:2000, Abcam-ab176752), Phalloidin-Alexa Fluor 555 (1:500, Sigma-P1951).

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